Synchronisierte Multiparameter-Messung kontraktiler Herzgewebe für das Wirkstoff-Screening
Eine dreimodale Plattform, die gleichzeitig Kalziumsignale, elektrische Aktivität und Kontraktionskraft in künstlichem Herzgewebe misst. Ein gemeinsames Zeitsteuerungssystem gewährleistet eine präzise Synchronisation und ermöglicht so detaillierte Analysen für die Prüfung von Herzmedikamenten und die Modellierung von Herzerkrankungen.
ADSYNX – Adaptive Control Method for Signal Synchronization
The ADSYNX technology provides a fast and adaptive method for the synchronization of different signals (skew compensation) for any electrical or optical system up to the GHz regime.
TomoSTED – faster STED imaging at lower light doses
Tomographic STED (TomoSTED) uses a rotating 1D depletion pattern instead of the classical 2D donut shaped STED-beam. The reduction from 2D to 1D depletion results in a more robust depletion with a favourable light intensity distribution pattern, which allows to significantly reduce the overall light dose while simultaneously increasing the signal to noise ratio. Thus TomoSTED allows faster imaging while causing less bleaching while maintaining image quality.
Reducing the mortality rate of patients after Aortic-Valve Implantation by Anti-Fibrotic Treatment
A subpopulation of patients with a very high risk to die in only one year after an aortic valve implantation (AVI) was identified. A novel, anti-fibrotic treatment was developed, which adresses the most important factor for the survival rate of patients and is being tested in a phase 3 clinical trial.
Fast deconvolution of 3D Light-Sheet-Images
VALID combines theoretical considerations and the usage of empiric image data to alculate the true dimensions of the a light sheet. This allows to deconvolve a 3D image stack by a precise subtraction of the bleed-through signal between the single slices at each position of the light sheet in 3D. Furthermore, VALID extracts information allowing to define the optimal distaince between the single slices during imaging, which reduces data size and speeds up imaging itself, as well as all subsequent image processing. This approach is surperior to known deconvolutuion methods, which are either made for pointwise (e.g. confocal) imaging or use crude estimations for the 3D shape of the lightsheet.
Multi Target Drugs for treatment of Heart Failure by stabilization of Ca2+ levels
Novel Multi-Target drugs, which can modulate two key players of the Ca2+ housekeeping in heart muscle cells were jointly developed at the University Medical Center Göttingen and the MPI-NAT. The substances show increased efficiency over comparable single target RyCal drugs, which are currently under investigation in clinical trials.
Novel small molecule HDAC-inhibitor: potential game changer to fight immune evasive cancers
A novel Inhibitor binding to a specific HDAC molecule reduces the ability of tumors to hide from the immune system and increases the ability of immune cells to attack tumors. This might widen the use of promising Antibody based tumor therapies also to those cancer types, where tumors evade from the immune system.
Dynamic Microconfiner: Biomimetic 3D cell culture
Novel Micronfiner for the first time allows to create a topological, mechanical and chemical controlled cell culture system for reproducible bio-mimetic culture conditions.
Mouse model for arthritic diseases
Scientists of the University of Göttingen developed a transgenic mouse model for sporadic Alzheimer’s Disease (tg4-42 = TBA83). This is the first mouse model without any mutations showing neurological deficits by transgenic over-expressing a major form of amyloid beta peptides (N-truncated Abeta 4-42) found in the brain of AD patients. Abeta 4-42 was the first peptide discovered in AD-typical plaques in 1986. The neurological phenotype resembles that of mouse models with neurodegeneration in the hippocampus.
Antibodies for Licensing
Chemokine receptors undergo internalization and desensitization in response to ligand activation. Internalized receptors are either preferentially directed towards recycling pathways (e.g. CCR5) or sorted for proteasomal degradation (e.g. CXCR4). Scientists at the Georg-August-University Göttingen developed a method monoclonal antibody against a biotinylable peptid (Epitope-Tag) called AP-tag or Avi Tag, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain. Anti-AP antibodies are useful tools in the analysis / trafficking of AP-tag fusion proteins, e.g. quantification of receptor-internalization from the cell surface into the cell. Binding of EF10 antibodies to the target protein is not affected by biotinylation of the acceptor sequence. Using these antibodies an alternative method was developed to analyse receptor internalization and recycling which allows a more detailed analysis of receptor trafficking compared to classical antibody-based detection methods. This biotin-based detection system may be generally applicable to the analysis of transmembrane protein trafficking.