Mouse model for arthritic diseases
Scientists of the University of Göttingen developed a transgenic mouse model for sporadic Alzheimer’s Disease (tg4-42 = TBA83). This is the first mouse model without any mutations showing neurological deficits by transgenic over-expressing a major form of amyloid beta peptides (N-truncated Abeta 4-42) found in the brain of AD patients. Abeta 4-42 was the first peptide discovered in AD-typical plaques in 1986. The neurological phenotype resembles that of mouse models with neurodegeneration in the hippocampus.
Antibodies for Licensing
Chemokine receptors undergo internalization and desensitization in response to ligand activation. Internalized receptors are either preferentially directed towards recycling pathways (e.g. CCR5) or sorted for proteasomal degradation (e.g. CXCR4). Scientists at the Georg-August-University Göttingen developed a method monoclonal antibody against a biotinylable peptid (Epitope-Tag) called AP-tag or Avi Tag, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain. Anti-AP antibodies are useful tools in the analysis / trafficking of AP-tag fusion proteins, e.g. quantification of receptor-internalization from the cell surface into the cell. Binding of EF10 antibodies to the target protein is not affected by biotinylation of the acceptor sequence. Using these antibodies an alternative method was developed to analyse receptor internalization and recycling which allows a more detailed analysis of receptor trafficking compared to classical antibody-based detection methods. This biotin-based detection system may be generally applicable to the analysis of transmembrane protein trafficking.
Algensammlung der Universität Göttingen (SAG)
Die Sammlung von Algenkulturen an der Universität Göttingen (SAG) ist eine der weltweit größten Sammlungen von lebenden mikroskopisch kleinen Algen. Gegründet in 1954, umfasst die SAG heute rund 2.250 Stämme aus ca. 521 Gattungen und 1202 Arten aus allen evolutionären Linien der eukaryotischen Algen und prokaryotischen Cyanobakterien. Den Hauptanteil stellen Stämme aus dem Süßwasser oder terrestrischen Lebensräumen, aber auch marine Algen und mikroskopische Stadien mancher Makroalgen stehen zur Verfügung. Eine Besonderheit bilden die Euglenophyta, da diese mit nahezu allen Gattungen (farbige und farblose) in der Sammlung vertreten sind. Im Katalog der Sammlung können alle Kulturen, die zur Verfügung stehen, gefunden werden mit Zusatzinformation wie Fundort, Reinheit, Nährmedien und Referenzen.
Animal model for Gastrointestinal Stromal Tumor (GIST)
Gastrointestinal stromal tumor (GIST) is one of the most common mesenchymal tumors of the gastrointestinal tract. GISTs occur in 10-20 per one million people. Thus, GIST has been named as an orphan drug disease. Scientists at the University of Göttingen developed a new animal drug screening model for GIST, which is caused by dysfunction of the Hedgehog pathway.
Animal model for sporadic Alzheimer’s Disease
Scientists of the University of Göttingen developed a transgenic mouse model for sporadic Alzheimer’s Disease (tg4-42 = TBA83). This is the first mouse model without any mutations showing neurological deficits by transgenic over-expressing a major form of amyloid beta peptides (N-truncated Abeta 4-42) found in the brain of AD patients. Abeta 4-42 was the first peptide discovered in AD-typical plaques in 1986. The neurological phenotype resembles that of mouse models with neurodegeneration in the hippocampus.
Monoclonal antibodies against biotinylable AP-tag / Avitag
Chemokine receptors undergo internalization and desensitization in response to ligand activation. Internalized receptors are either preferentially directed towards recycling pathways (e.g. CCR5) or sorted for proteasomal degradation (e.g. CXCR4). Scientists at the Georg-August-University Göttingen developed a method monoclonal antibody against a biotinylable peptid (Epitope-Tag) called AP-tag or Avi Tag, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain. Anti-AP antibodies are useful tools in the analysis / trafficking of AP-tag fusion proteins, e.g. quantification of receptor-internalization from the cell surface into the cell. Binding of EF10 antibodies to the target protein is not affected by biotinylation of the acceptor sequence. Using these antibodies an alternative method was developed to analyse receptor internalization and recycling which allows a more detailed analysis of receptor trafficking compared to classical antibody-based detection methods. This biotin-based detection system may be generally applicable to the analysis of transmembrane protein trafficking.