Aberration correction for STED microscopy

Written by Dr. Martin Andresen on 07 September 2020.

Fast and sample-friendly aberration correction for STED microscopy

A simple metric, based on local changes in the fluorescence lifetime was found to reflect the quality of the STED focus. This metric can be used to correct abberations fast, on-line and continuously over the whole image, using a simple deformable mirror and an elaborated algorythm.

Aberration correction for STED microscopy

Written by Dr. Martin Andresen on 07 September 2020.

Fast and sample-friendly aberration correction for STED microscopy

A simple metric, based on local changes in the fluorescence lifetime was found to reflect the quality of the STED focus. This metric can be used to correct abberations fast, on-line and continuously over the whole image, using a simple deformable mirror and an elaborated algorythm.

Tomographic STED reduced light doses

Written by Dr. Martin Andresen on 07 September 2020.

TomoSTED - faster STED imaging at lower light doses

Compared to classical STED Microscopy, the Tomographic STED (TomoSTED) uses a rotating 1D depletion pattern instead of the classical 2D donut shaped STED-beam. A favourable intensity distribution and increased stability allow allows faster imaging AND less bleaching.