New approach for Ligh-Sheet-Deconvolution

Written by Dr. Martin Andresen on 29 March 2022.

Fast deconvolution of 3D Ligh-Sheet Images (VALID)

Vertical Algebraic LIght sheet Deconvolution (VALID) combines theoretical considerations and the usage of empiric image data for deconvolving 3D image stacks. By using an iterative approach, VALID is able calculate the true dimensions of a light sheet, which allows to precisely subtract bleed-through signal between the single slices - at each position of the light sheet in 3D.